The research aimed to reveal the acquaintance level of the natural science secondary stage teachers with the concepts and applications of nanotechnology and the attitudes of their students towards it. The descriptive approach was used; and a test was designed to measure the level of acquaintance with the concepts and applications of nanotechnology among a sample of natural science secondary stage teachers in Bisha city, which reached (46) teachers; and a measure of their students' attitudes toward it on a sample of (460) students. The results of the (T) test for one group indicated a low level of knowledge of natural sciences secondary stage teachers with concepts and applications of nanotechnology, and the existence of positive attitudes for secondary school students towards those concepts and applications. The results of the ANOVA and Scheffe test also indicated that there are statistically significant differences between the mean scores of the teachers in the test of the level of familiarity with the concepts and applications of nanotechnology due to the variable of specialization in favor of physics teachers and the variable of experience in favor of teachers with (11) years of experience and more. In light of the results, the research presented a proposed perception to develop the level of familiarity of natural sciences secondary stage teachers with concepts and applications of nanotechnology in the form of an electronic training package.